Study of MAIT Cell Activation in Viral Infections In Vivo

TSC Hinks; B van Wilgenburg; H Wang; L Loh; M Koutsakos; K Kedzierska; AJ Corbett; Z Chen

Journal article

Study of MAIT Cell Activation in Viral Infections In Vivo

TSC Hinks, B van Wilgenburg, H Wang, L Loh, M Koutsakos, K Kedzierska, AJ Corbett, Z Chen

Methods in Molecular Biology | HUMANA PRESS INC | Published : 2020

DOI: 10.1007/978-1-0716-0207-2_17

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Abstract

MAIT cells are abundant, highly evolutionarily conserved innate-like lymphocytes expressing a semi-invariant T cell receptor (TCR), which recognizes microbially derived small intermediate molecules from the riboflavin biosynthetic pathway. However, in addition to their TCR-mediated functions they can also be activated in a TCR-independent manner via cytokines including IL-12, -15, -18, and type I interferon. Emerging data suggest that they are expanded and activated by a range of viral infections, and significantly that they can contribute to a protective anti-viral response. Here we describe methods used to investigate these anti-viral functions in vivo in murine models. To overcome the tec..

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University of Melbourne Researchers

Huimeng Wang Author

Liyen Loh Author

Marios Koutsakos Author

Katherine Kedzierska Author

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Awarded by University of Melbourne

Funding Acknowledgements

This work was funded by grants to T.S.C.H. from the Wellcome Trust (104553/z/14/z, 211050/Z/18/z) and Project Grants 1062889 and 1120467 and Program Grant 1113293 from the National Health and Medical Research Council of Australia. B.W. was supported by the Royal Society (IE160540). A.J.C. is supported by a Future Fellowship from the Australian Research Council, FT1600100083. The research leading to these results has received funding from the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA grant agreement number 608765. The content represents only the authors' views and not those of the European Commission. HW was supported by a Melbourne International Engagement Award (University of Melbourne).